Operation, Calibration, Cleaning and Maintenance of UV Cabinet

 

1.0	OBJECTIVE
		To lay down the Procedure for Operation, Calibration, Cleaning and Maintenance of UV Cabinet.
2.0	SCOPE
		This Standard Operating Procedure is applicable for Operation, Calibration, Cleaning and Maintenance of UV Cabinet bearing details.
3.0	RESPONSIBILITY
	3.1	Research Associate/Executive – ADL shall be responsible for activities as per SOP.
	3.2	Manager / Designee ADL - shall be responsible to ensure activities are performed as per SOP.
	3.3	Head - Research Centre (RC) - shall be responsible for implementation of system and     authorization of SOP.
4.0	PROCEDURE
	4.1	Operation:
			Ensure that the UV cabinet and its observation glass window, which is situated on the top of the instrument is clean.
			Plug the cord of UV cabinet to power supply outlet and switch on the mains.
			Put ‘ON’ the Main switch of the instrument situated at the side of the instrument.
			Open the front door of the UV cabinet and place the TLC plate / sample under test in the UV cabinet hood and close the door properly. UV cabinet can operate without timer and with timer mode. Operation of UV Cabinet without Timer: Set the required wavelength ( at which sample is to be observed) by controlling toggle switch  ‘254 nm’ switch for UV  short  region  or ‘366 nm’ switch UV long region  and allow  to stabilize for 10 minutes. Observe the TLC plate / sample through the glass window and note down the observations. Put ‘OFF’ the wavelength switch and remove the TLC plate / sample from the sample hood. Switch off the instrument and mains. Operation of UV Cabinet by using Timer: Switch ‘ON’ Timer switch by using timer controlling toggle switch. Display will show presetted time.  To set the time press <SET> push key, digits will flash on display. To change the digit press <Start/Stop> key. To change or to shift to the next digit again press <SET> key. If presetted time is acceptable then switch on the required light source switch. Source will be ignited. Press start/stop push key on panel. Source will ignite & Timer display will start descending. When display will come to ’00.0’ automatically source will be switched OFF & again display will show presetted value. To select another source, switch ‘ON’ the corresponding wavelength selector switch & again press <Start / Stop> push key on panel. If source has to be switched ‘OFF’ before elapsed time again press same start / stop push key. 4.2 Calibration 4.2.1 Solution 1: Weigh accurately about 40 mg of Sodium salicylate in 100 mL volumetric flask and dilute to 100mL with ethanol. 4.2.2 Solution 2: Weigh accurately about 200 mg of Sodium salicylate in 100 mL volumetric flask and dilute to 100 mL with ethanol. 4.2.3 Procedure: Apply 5 µl each of following solution 1and 2 on the plate coated with silica gel G separately and observe the spot after development under UV light at 254 nm for solution 1 and at 365 nm for solution 2. 4.3 Cleaning: 4.3.1 Ensure the UV Cabinet is clean and calibrated. 4.3.2 Do not keep the door of the instrument open. 4.3.3 Whenever require clean the system of outside surface with dry cloth. 4.4 Maintenance 4.4.1 If the instrument does not produce required results or it’s response is poor then it should be brought to notice of the Manager ADL and the instrument should be repaired from suitable source. ABBREVIATIONS UV: Ultra Violet TLC: Thin Layer Chromatography QA: Quality Assurance SOP: Standard Operating Procedure RC: Research Centre ADL: Analytical Development Laboratory mL: milliliter nm: nanometer µL: microliter 5.0 FREQUENCY  5.1 NA 6.0 ACCEPTANCE CRITERIA 6.1 The lamp is capable of revealing without doubt standard spots of Sodium salicylate. 7.0 RELATED DOCUMENTS 7.1 Nil 8.0 REFERENCES 8.1 Operating Manual